Journal: Frontiers in Immunology

Article Title: Obesity Prolongs the Inflammatory Response in Mice After Severe Trauma and Attenuates the Splenic Response to the Inflammatory Reflex

doi: 10.3389/fimmu.2021.745132

Figure Lengend Snippet: List containing all antibodies utilized for surface staining of mass cytometry samples.

Article Snippet: 155Gd , CD206 , MR5D3 , FisherScientific , 13246099 , 1:100.

Techniques: Staining, Mass Cytometry, Marker

Journal: Folia Microbiologica

Article Title: Detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification

doi: 10.1007/s12223-011-0072-7

Figure Lengend Snippet: Analytical specificity of the LAMP was observed using agarose gel electrophoresis. Lane M 2,000–100 bp ladder marker (50 U/μl; Protech, Taiwan); lanes 1–7 extracted DNA of PCV1, PCV2, PPV, PRV, cDNA of CSFV, PRRSV, and negative control, respectively

Article Snippet: Lane M 2,000–100 bp ladder marker (50 U/μl; Protech, Taiwan); lanes 1–7 extracted DNA of PCV1, PCV2, PPV, PRV, cDNA of CSFV, PRRSV, and negative control, respectively By testing 10-fold serial dilutions (10 7 copies/μl to 1 copy/μl) of the recombinant plasmid, the LAMP demonstrated a detection limit of 10 copies (Fig. , lane 7), comparable to the nested PCR (10 copies; Fig. , lane 15).

Techniques: Agarose Gel Electrophoresis, Marker, Negative Control

Journal: Folia Microbiologica

Article Title: Detection of porcine circovirus type 1 in commercial porcine vaccines by loop-mediated isothermal amplification

doi: 10.1007/s12223-011-0072-7

Figure Lengend Snippet: Analytical sensitivities of the LAMP ( lanes 1–8 ) and the nested PCR ( lanes 9–16 ) were compared by detecting 10-fold serial dilutions of the recombinant plasmid (10 7 copies/μl to 1 copy/μl) by agarose gel electrophoresis. Lane M 2,000–100 bp ladder marker (50 U/μl; Protech, Taiwan). The PCR products of lanes 9–15 were 155 base pairs (bp) in length

Article Snippet: Lane M 2,000–100 bp ladder marker (50 U/μl; Protech, Taiwan); lanes 1–7 extracted DNA of PCV1, PCV2, PPV, PRV, cDNA of CSFV, PRRSV, and negative control, respectively By testing 10-fold serial dilutions (10 7 copies/μl to 1 copy/μl) of the recombinant plasmid, the LAMP demonstrated a detection limit of 10 copies (Fig. , lane 7), comparable to the nested PCR (10 copies; Fig. , lane 15).

Techniques: Nested PCR, Recombinant, Plasmid Preparation, Agarose Gel Electrophoresis, Marker